All of these embryos expressed eGFP and 15 embryos developed to blastocyst stage. Of 4,951 porcine SCNT-TG embryos generated, 850 were cultured in the presence of 1 µg/ml doxycycline in vitro. The Tet-on system was then tested in the generated porcine SCNT-TG embryos. Somatic cell nuclear transfer (SCNT) was then performed using these donor cells. At 1 day after treatment with 1 µg/ml doxycycline, the fluorescence intensity of these cells was increased. Porcine fetal fibroblasts were infected with a universal doxycycline-inducible vector containing the target gene enhanced green fluorescent protein (eGFP). The Tet-on system was first tested in infected donor cells.
The aim of this study was to produce pigs expressing a tetracycline (Tet)-inducible transgene. Transgenic (TG) pigs are widely used as models of human diseases.
Pigs are anatomically and physiologically closer to humans than other laboratory animals.
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